There are many portraits of unknown ladies that date from the time of Queen Claude of France.
These portraits are very realistic, being of the school of Clouet. Are any of them suspected of being Anne Boleyn? I suspect that Anne might have been painted by Hans Holbein, and that any portrait of her was destroyed after her beheading. Henry seemed to hate her as much as he loved her and tried to erase her from his past life.
Why hasn't someone unearthed the bodies buried under the floor of St Peter ad Vincula? Forensic reconstruction of Catherine Howard, Jane Grey and Anne would be a wonderful boon to Tudor history fanatics around the world.
[Note from Lara - many discussions about the burials in St. Peter ad Vincula has already occurred in past threads, so I would just refer you all to the search box to the left for more on that topic.]